Evaluation of Virucidal Efficacy of Cold Plasma on Bacteriophage Inside a Three-Layered Sterilization Chamber

Traditional disinfection methods against pathogens have numerous shortcomings, and inventive like cold plasma are required for virus inactivation. Here, an atmospheric pressure 13.56 MHz radio-frequency hollow cathode (RF–HC) device has been used to build a sterilization device, its virucidal activity is assessed P2 bacteriophage, model surrogate pathogenic viruses. The heart of this contains three-layered chamber which rectangular parallelopiped 42 × 32 30 cm3. Optimization experiments were performed make each corner completely free after treatment. This two-pronged study was conducted establish the requirement minimum vol. % H2O2 in time complete elimination phages inside even when not direct line sight. In initial experiments, effect sight seen as top bottom layers showing less phage killing compared middle chamber. Complete all three chamber, achieved by treatment with 6% 10 min 80 watts operating power. It also that mist alone sufficient provide high degree sterilization, normal water combined can higher level at